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An indirect capture model assay using colloidal Au nanoparticles is demonstrated for surface enhanced Raman scattering (SERS) spectroscopy detection of DNA.
Therefore, a model assay was developed in which fluorophore-loaded unilamellar vesicles were permeabilised by soluble oligomers, the latter formed by aggregation of human recombinant αS protein.
The reversibility of the fluorescence response is demonstrated by means of an enzymatic model assay using ATPase for the decomposition of adenosine triphosphate.
The third model (assay removal, Additional file 1: Figure S1C) removed whole assays at random and was only applied to the PKIS data set, as the number of assays in the HTSFP subsets was deemed too small.
Here, we describe a successful model assay targeting 24 HPV genotypes most commonly associated with the progression to cervical cancer.
The in vivo rabbit eye model assay showed that apoEdp suppressed VEGF-induced angiogenesis in a corneal micro-pocket assay.
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A structure activity relationship is described for a series of peptides which were synthesised and tested for their activity against two prion disease model assays, an in vitro cellular assay and an in vitro anti-aggregation polymerisation assay.
Compounds that showed promising COX-2 selectivity were further subjected to in vivo anti-inflammatory screening applying formalin induced paw edema (acute model) and cotton-pellet induced granuloma (chronic model) assays using celecoxib and diclofenac sodium as reference drugs.
An orthotopic mouse model assayed ability to produce tumours in vivo.
We are now developing mouse model assays to further characterise the role of the CCL20 CCR6 axis in Treg cell homing in vivo.
Moreover, the effects of up-regulating NDRG2 expression on the growth of an ESCC cell line were examined by MTT, colony formation, DNA replication activity and nude mouse model assays.
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