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We utilized a dose of 12 mg/kg/day in order to obtain a similar reduction in blood pressure as is achieved using losartan in our Marfan mouse model, as we wished to eliminate blood pressure as a confounding variable when comparing the differential physiological and biochemical effects of the two agents.
SEM usually requires large samples, but required sample sizes depend on various characteristics of the model as well as what differences one wishes to detect in the analyses.
We also modeled PSS score as a continuous function of medium-term moving average exposure of predicted PM2.5 and BC as estimated from the spatio-temporal models, as we wished to evaluate whether the associations for predicted PM2.5 and BC were consistent with those exposures measured from the stationary monitoring site.
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