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For those used as the basis for the Calgary/Edmonton tour-based freight vehicle model, an alignment between the total employment by industry category in the case study area and in the region was achieved [9].
We further refined sequence search by using hmmer 3.0 (http://hmmer.org/) to create a hidden Markov model – an alignment profile for more sensitive sequence search.
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Besides the ligand-based CoMFA models, an alignment molecular set constructed by flexible molecular docking has been also studied.
The decoding methods that use the SFF model produce an alignment and a repeat annotation.
Thus, this model assumes an alignment of resources, which clearly does not always occur.
This may be because that MODELLER cannot build a reasonable model from an alignment with too many errors.
The 1T-1DCDN primer was designed with the sequence 5' TCT ACT GGR TYR ACR TG 3' using the primer 1T-1D [ 38] as a model, and an alignment of the VP4 variable region using the Clustal W algorithm in MEGA 5.0 [ 41].
However, the relatively small genetic distances detected (dS and dI < 0.05) suggest that the p-distances calculated in table 2 should also be accurate despite the transition bias [ 25]. Figure 1 shows a monophyletic neighbour joining tree, deduced using Kimura's two parameter model from an alignment of ~30,000 bases and 1,800 variable sites (additional file 2).
Profile HMMs are probabilistic models representing an alignment of multiple amino acid sequences that are very effective at identifying related sequences [ 52].
In the outer level of the model, we optimize the horizontal alignment and in the inner level of the model a vertical alignment optimization problem considering earthwork allocation is solved for a fixed horizontal alignment.
Using these putative exons as a model, a sequence alignment was produced such that each intron concurred with the GT/AG intron donor/acceptor site rule [ 27].
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