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MG1655 genome sequence (Genbank accession NC_000913), using the Novoalign software package (Novocraft, Selangor, Malaysia) in single-end read mode with default parameters.
We used our 3PD program in 'Targeted Mode' with default parameters.
SSR motifs were searched on SME_r2.5.1 by using the SciRoKo software in the 'MISA' mode with default parameters.
The Ion Torrent Variant caller (version 4.2.0) was used in "Germline - low stringency" mode with default settings.
Transfer RNA genes were predicted using tRNAscan-SE, version 1.4, in a eukaryotic mode with default parameters.
misSEQ uel performs this step using BWA (version 0.5.9) in paired-end mode with default parameters (Li and Durbin 2009).
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Mutation Surveyor software (MS; Softgenetics, State College, PA) version 4.0.5 was used in batch-mode with default parameters to align ab1 files to target reference sequence and make genotype calls.
The standard precision (SP) docking mode, with the default parameters, and the Glidescore scoring function, both recommended for virtual screening, were used.
UProC was used in short read (best hit) mode with the default noise threshold (0.1% FPR).
We ran MuTect under the High-Confidence mode with its default parameter settings.
The ventilator was set up in a controlled mode with the default front screen visible with capnography disabled.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com