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SR XRD (Synchrotron radiation X Ray diffraction) patterns in transmission mode were acquired at the beamline ID18F of the ESRF.

During the TEM analysis of the nanohole, both bright-field TEM (BF-TEM) images in conventional TEM mode were acquired, as shown in Fig. 4a, b, as well as high-angle annular dark field images in scanning TEM mode (HAADF-STEM), as shown in Fig. 4d, e, in order to compare the measurement accuracies.

ESI-MS spectra (positive or negative mode) were acquired from m/z 50-1200 for 2.5 min. Operating conditions were as follows: accumulation time, 300 ms; dry temperature, 350 C; capillary voltage, 3500 V; nebulizer, 30 psi; dry gas, helium at 8 L/min.

In order to enhance the detection of putative phosphorylated species, MALDI-TOF spectra in the negative, linear mode were acquired from H1 after digestion with AspN.

High-resolution mass spectra (resolution Δ(m/z)/(m/z) of 500.000 at m/z 500 in full scan mode) were acquired on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (Bruker, Bremen, Germany), equipped with a 12 Tesla superconducting magnet and an Apollo II ESI source.

Electron impact spectra in positive ionization mode were acquired between m/z 40 and 450.

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A second scan of the same region in CTM mode was acquired at a table speed of 0.6 mm/s.

The adult-type maintenance mode is acquired in the first years of life, correlating with a drastic increase in mtDNA copy number.

At each bed position, a 5-min emission scan in three-dimensional mode was acquired.

Before performing a tandem mass spectrum, an ESI/MS mass spectrum (TOF MS mode) was acquired for each HPLC fraction over the mass range of 100 2000  m/ z, in order to select the ion of interest; subsequently, these ions were fragmented in the collision cell (TOF MS/MS mode).

Complete performance curves of its pump and turbine modes were acquired.

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