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The ions generated in the negative ion mode were accumulated in the hexapole region for 0.8 s and transferred subsequently into the ion cyclotron resonance (ICR) cell.
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Ions were generated with an Apollo pneumatically assisted electrospray ionization source (Analytica) operating in the negative ion mode to detect sulfolipids or the positive ion mode to detect PDIM and were accumulated in an RF-only external hexapole for 1 s before being transferred to the ICR cell for mass analysis.
In MS/MS positive ion mode, for one main MS spectrum, 50 subspectra with 50 shots per subspectrum were accumulated using a random search pattern.
Eight scans were accumulated for each titration.
Two hundred fifty six scans were accumulated for each spectrum.
Typically, 1600 shots were accumulated for spectra.
1000 scans were accumulated.
64 transients were accumulated.
For the first detection step (so-called "survey scan") the single quadrupole "enhanced" MS mode (EMS), where ions are accumulated then filtered in the Q3-linear ion-trap, was used.
Bugs were accumulating.
We can see that the primary throughput in cooperation mode η C P is always higher than that in the conventional primary system ηP; thus, credits can be accumulated by the secondary system in cooperation mode.
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