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LC/ESI MS/MS was performed in negative ion mode using multiple reaction monitoring mode.
The detection was obtained in ESI positive mode using multiple reaction monitoring (MRM), with transition channels of proton molecular ions was m/z 376.05 > 250.97 for linifanib and m/z 399.12 >283.02 for IS, having dwell time of 0.106 s.
Ions were detected in a positive ion mode using multiple reaction monitoring.
In brief, TBBA and TIBA were analyzed in negative electrospray ionization mode using multiple reaction monitoring.
Electrospray ionization interface was used with the source temperature of 250°C, operating in positive mode using multiple reaction monitoring (MRM).
The instrumental systems comprised a Shimadzu Prominence LC with a Phenomonex Luna Hilic (100 × 2.00 mm) column and an AB Sciex API 4000 triple quadrupole mass spectrometer operated in electrospray ionization positive ion mode using multiple reaction monitoring (MRM) detection.
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For DFC quantification mass spectral data were acquired in positive ion electrospray ionization (ESI) mode using the multiple reaction monitoring (MRM) scan mode.
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