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GSLs were analyzed in the positive ion mode using glycerol as matrix.
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The enzyme activity was determined by titration using glycerol tributyrate (tributyrin) as the substrate in a pH-stat mode at 30°C in a 0.005 mol/L Tris HCl buffer (pH 7.5) containing 0.025 mol/L CaCl2.
Fastest growth was achieved at 30 °C, using glycerol as solute.
In this study, we designed the intraperitoneal glycerol tolerance test (IPGlyTT) and studied glycerol tolerance in vivo using glycerol kinase (Gyk) carrier (C) and wild type (WT) mice.
Slides were then mounted using glycerol and covered with coverslips.
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The $3.6 million-a-year effort is aimed at developing catalysts and other technologies that will use glycerol as a feedstock "for making various downstream chemicals," Mr. Simons said.
Moreover, T. lutea used glycerol only in mixotrophic conditions and no use of glycerol occurred in heterotrophic conditions (Fig. 1b).
The optimal conditions determined using pure glycerol (commercial grade glycerol), were successfully applied to the fermentation of crude glycerol from biodiesel production.
Medium was sampled at the indicated times, and glycerol was measured as an index of lipolysis by using free glycerol reagent (Sigma) against a glycerol standard curve.
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