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Quantitative PCR (qPCR) is usually performed in real-time mode using fluorescence detection methods.
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The fluorescence was read using fluorescence microscope.
Proteasome activity was determined using fluorescence assays.
A minimum of 5000 events was computed in list mode using log-amplified fluorescence signals and linearly amplified side- and forward-scatter signals.
For polyP-NOL substrate, absorbance at 400 nm was measured using a SpectraMax M2 microplate reader (Molecular Devices; Sunnyvale, CA); for polyP-MU substrate, fluorescence was quantified in fluorescence mode using excitation at 360 nm, emission at 450 nm, and a 435 nm cutoff filter.
Area detection of the fluorescence intensity was acquired in reflection mode using a 780 nm fluorescence filter and standard 50 mm lens (Nikon) mounted to a microchannel plate (Picostar HRI, La Vision) for signal amplification, which was coupled to an electron-multiplying CCD camera (Andor) to capture the image.
The XANES spectra of Ni + Zn Alox (Alox refers to Al oxide), Zn Alox, and Zn + Ni Alox were collected in fluorescence mode using Lytle detector, whereas the XANES of Ni Alox at pH 6.0 was collected in fluorescence mode using a 13-units multi-element Germanium Detector.
Spectra were collected in fluorescence mode using a 13-element germanium array detector.
The spectra were collected in fluorescence mode using a silicon drift detector.
XANES spectra were measured in fluorescence mode, using a passivated implanted planar silicon detector.
The spectra were collected in fluorescence mode using a Lytle detector, which was filled with pure Ar2 and positioned at a 90° angle to the incident beam.
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