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Chromatographic separation was accomplished using reversed-phase liquid chromatography or hydrophilic interaction liquid chromatography with gradient elution and detection by tandem mass spectrometry in the positive ionization mode using electrospray ionization.
After elution the column was washed for 10 min with 100% RP buffer B and equilibrated for 10 min with 100% RP buffer A. The LTQ mass spectrometer (ThermoFisher Scientific, San Jose, CA) was operated in the positive ion mode using electrospray ionization with a capillary temperature of 200°C.
Here, the instrument was operated in Q1 full-scan negative ion mode using electrospray ionization.
The molecular mass andpeptide sequencing were estimated by positive ion mode using electrospray ionisation-mass spectrometry.
The mass spectrometer was operated in positive mode using electrospray ionisation with a voltage of 1400 V.
The instrument was operated in positive ion mode using electrospray ionization with a spray voltage of 4.5 kV and a capillary temperature of 225 °C.
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Detection was performed under the multiple reaction monitoring (MRM) mode using an electrospray ionization (ESI) in the positive ion mode.
The amino acids were detected using a Micromass Quattro Micro tandem mass detector in MRM mode using positive electrospray ionization, with the following transitions: ornithine, 133.1 → 70.2, arginine 175.2 → 70.2 and citrulline 176.2 → 70.2.
Most compounds were analyzed using electrospray ionization in the negative mode, except -1, which was analyzed using positive mode.
Ionization was achieved using electrospray in positive ionization mode (ESI+).
Detection was performed on tandem mass spectrometer using electrospray ionization source in positive mode by multiple reaction monitoring.
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