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The data were collected in fluorescence excitation mode using a Lytle detector.
Single plane images were acquired on a Zeiss Airyscan 880 microscope in confocal mode using a 20× objective.
Uniquely aligned reads were counted using htseq-count (intersection -strict mode) using a Homo sapiens annotation table (GRCh38.79.gtf, http://www.ensembl.org).org
The energy of the X-ray beam was tuned by a double-crystal monochromator operating in fixed-exit mode using a Si (111) crystal pair.
Briefly, electrospray ionization (ESI) -spectra were acquired in the positive ion mode using a Waters LCT Premier instrument equipped with a TOF analyser.
a) Images for tdTomato were acquired in conventional confocal mode using a Cy3 conjugated secondary antibody and a 561 nm excitation laser line (AOTF = 7%).
The chromium decontamination was then optimized in a batch mode using a central composite design (CCD).
Selection of working mode using a switch provides flexibility for current-mode design.
Operation was conducted in pulsed mode using a nominally 2.25 MHz, 15 mm diameter transducer.
The controller alleviates the chattering phenomenon associated with the sliding mode using a boundary-layer technique.
Subsequently, the device was tested in passive mode using a mixture of 5 M MeOH +0.5 M KOH.
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