The 9-hole plate was removed and an 11-hole anterior plate was applied and tested, again using three 3.5 mm locking screws in unicortical lock mode on either side of the lateral plate gap.
Cells were then processed for confocal microscopy imaging, which was performed in multi-tracking mode on either a Zeiss LSM510 or a Zeiss LSM510 META.
All spectra were acquired in positive ion mode on either an Autoflex Speed TOF MS or an Ultraflextreme TOF/TOF MS (Bruker Daltonics), both equipped with a Nd YAG (solid state) laser operating at 355 nm.
Whole-body scans extending from base of skull to mid-thigh were obtained in 2-D mode on either the GE-supplied PET Advance scanner or Discovery-ST PET/CT scanner.
Mass spectrometric experiments were performed in positive ion mode on either a quadrupole quadrupole time-of-flight (quadrupole quadrupole time-of-flightty, CA, USA) or a quadrupole quadrupole time-of-flightp mass spectrometer (QqTOF; QSTAR 3200, AB ScieXL.
LC-MS and LC-MS/MS of reaction products was performed in positive mode on either a Thermo Fisher (Waltham, MA) Orbitrap XL or a Thermo Fisher Orbitrap Fusion utilizing short linear gradients from 0.1% formic acid in water to 0.1% formic acid in 80% acetonitrile.
