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Nonetheless, these data suggest that the dominant theme for nucleosomes on CRM1, CRM2 and CentC elements (as well as the genome as a whole) is spacing intervals of ~190-bp on average - though the mode of positioning and rigidity of the chromatin depends on the local DNA.
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As our analysis is conducted in YPD medium, it is very interesting to investigate whether this regulatory mode of nucleosome positioning is conserved in other cellular conditions.
Taken together, we identified distinct regulatory modes of nucleosome positioning.
The origins and consequences of different regulatory modes of nucleosome positioning also remain to be elucidated.
For CENH3-containing nucleosomes, distinct modes of nucleosome positioning were evident within that general spacing constraint.
These three different DNA elements, however, were sharply distinguished from each other by modes of nucleosome positioning.
We identified four different regulatory modes of nucleosome positioning and gave insights into mechanisms that specify promoter nucleosome location.
Using multiple sources of data generated in YPD medium, we identified four typical promoter classes characterized by distinct regulatory modes of nucleosome positioning.
We identified four typical gene classes associated with distinct regulatory modes of nucleosome positioning, and further showed that they are distinguished by transcriptional regulation patterns.
In summary, these results indicate that centromere DNA elements influence chromatin structure but leave open the question of whether one or more of these distinct modes of nucleosome positioning have consequences in terms of centromere specification and kinetochore function.
We further identified four distinct regulatory modes of nucleosome positioning: DNA-encoded open nucleosome (nucleosome-depleted) organization, nucleosome eviction, nucleosome sliding, and non-DNA-driven closed nucleosome (nucleosome-enriched) organization.
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