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Furthermore, our chimeric analyses revealed that FBM neurons have an alternative, collective mode of migration that requires interactions between migrating FBM neurons themselves and occurs independently of Nhsl1b or PCP proteins in the migrating neurons.
Furthermore, a "collective mode" of migration that requires the interaction between migrating facial BMNs themselves and is independent of PCP proteins has been suggested to work together with PCP-dependent mechanisms to drive directed migration of facial BMNs in vivo (Walsh et al., 2011).
We observed that vangl2, scrib and nhsl1b mutant FBM neurons, which are unable to migrate using the 'PCP-dependent' mode of migration, can be 'rescued' in their migration if they are in the presence of neighboring wild-type FBM neurons.
Time-lapse microscopy to exemplify the mode of migration of a GFP-expressing rat Schwann cell migrating along a tubule of HUVECs within a 3D fibrin gel.
The model based on Rakic, 1988 (Science, 241 170) emphasizes the radial mode of migration which underlies prominent columnar organization in primates.
The amoeboid mode of migration that applies to E. histolytica's displacements on two-dimensional surfaces is also expected to apply to the three-dimensional environment in the human intestine although several unknown, distinct modalities may be involved.
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It should be noted that, in the Express Train Hypothesis, there are two different aspects: 1) the origin of the migrations, i.e. the Taiwan Homeland Hypothesis, and 2) the mode of migrations, i.e., a rapid dispersal starting from Indonesia.
Importantly, these 3D modes of migration provide insight into the potential for oncogene-transformed cells to migrate within and colonize tissues of varying stiffness.
Metastatic cells switch between different modes of migration through supramolecular plasticity mechanism(s) still largely unknown.
Numerous previous studies have focused on the roles of different microenvironmental characteristics and how they affect tropism and cancer dissemination2,3,4,5,6, but their results have not explained why different cancers exhibit different modes of migration.
Further explorations of the commonalities and differences between distinct modes of migration will enhance our understanding of how neurons reach their final positions during development.
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