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Experimentally, we encounter this sharp increase of the number of generated molecules around levels 9 through 11, which can be expected considering the mode in the distribution of molecular fragments in the DUD-E database presented in Fig. 4.
The data were centered by the tallest mode in the distribution of the segment values.
Indeed, the second mode in the distribution of pairwise nucleotide differences was larger between population (π b ) than that of within population (π w, Figure 1A).
We find a significant increase in the variance of the first mode in the distribution of pairwise nucleotide differences between 2005 and 2009 (p < 0.01 two-sided Bartlett test).
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In brief, the larger the t, the larger the difference between the two peaks (i.e. modes) in the distribution of a certain gene signal profile within the cohort.
This mode-shift in the distribution of allele frequencies in WB and PB signifies population bottlenecks.
The presence of a mosaic blocks pattern and evidences for a mode-shift in the distribution of allele frequencies found in WB and PB agrees with theoretical expectations for populations which have undergone a severe bottleneck [ 8].
We show, for example, that the number, size, and dynamics of modes in the distributions of the pairwise nucleotide differences in populations are informative on the existence of past isolation.
A mode-shift distortion in the distribution of allele frequencies was evident.
We performed simulations with the software BOTTLENECK 1.2.02, assuming an infinite allele mutation model searching for mode-shift distortion in the distribution of allele frequencies.
Author's response Population bottlenecks cause a characteristic mode-shift distortion in the distribution of allele frequencies at selectively neutral loci.
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