Ions were generated by ESI source and analyzed by multiple reaction monitoring mode (basis for quantification) in the Agilent 6410 QqQ analyzer.
Peak identification: 1. Pachycarin A, 2. 3′,4′-dimethoxy(2′′,3′′:7,8) furanoflavone, 3. Karanjin, 4. Karanjachromene, and 5. Isoderricin A. The positive ionization mode was selected for quantification tests.
Positive multiple reactions monitoring (MRM) mode was chosen for quantification of each analyte and cystamine dihydrochloride (CYA) was used as the internal standard (IS) for the assay.
Multiple Reaction Monitoring (MRM) mode was performed for quantification.
The multiple reaction monitoring (MRM) mode was chosen for quantification of the metabolites of the probe substrates (Table 1).
Selected reaction monitoring (SRM) mode was used for quantification: m/z 253.1 → 159.1 for cimetidine and m/z 329.1 → 256 for tiapride.
SRM mode was used for quantification: m/z 329.1 → 284.9 for furosemide and m/z 315.1 → 270.9 for 4-hydroxydiclofenac- d4.
Using either mode of quantification, 103+/ kasA bacteria multiplied significantly less in macrophages than 103+.
To cover the broad suite of organic analytes of interest, we used three different extraction methods, each combined with GC/MS in the multiple ion detection mode, for detection and quantification.
The effectiveness of using frequencies and both frequencies and mode shapes as the data for quantification of damage extent are examined.
