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Mass spectra of the urine mixtures were recorded in the total ion mode for identification of components and for selection of substances for quantitative analysis.
Each digest was analyzed in LC-MS mode for identification.
The peptides were then extracted from the gel and placed on an AnchorChip 600 plate for use in a MALDI-TOF/TOF AutoFlex III™ mass spectrometer (Bruker Daltonics, USA), in positive reflector mode, for identification.
Samples were measured both in linear mode, providing information on the total number of different structures, and in reflector mode, for identification of molecular formulas based on precise mass measurements.
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This identification technique was used because of its high sensitivity, small injection volumes, ultralow flow rates, resulting in the low consumption of hazardous chemicals, and the option to operate in full scan accurate mass mode for the identification of unknown compounds based on elemental composition calculated thereof.
In HMMER package, 'ls' mode is suitable for identification of a complete sequence domain, while 'fs' mode is capable of finding domain fragments and maybe useful to detect incomplete rRNA genes.
After morphologic evaluation, color Doppler mode was activated for identification of vascular color signals.
In this concept, nano-LC-Q-ToF in full scan accurate mass mode was used for identification instead.
GC-MS/MS (Agilent 7000 and Agilent 6890N with Waters Micromass MS) in multiple reaction monitoring mode was used for identification and quantification of 209 PCB congeners as 159 chromatographic peaks.
As the new method is a FRF-based method, it overcomes the problem of closely spaced modes for damping identification.
This is particularly true with the increasing need to more rapidly detect associations across various information modes for threat identification and determination in real-time, security-related contexts for example, in situations involving time critical targets of national importance where rapid assessments must be made as to the type and degree of threat that may or may not exist.
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