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The LTQ Orbitrap XL mass spectrometer was run in data-dependent acquisition mode using Xcalibur 2.2 software (Thermo Scientific) using the positive ion mode for data acquisition.
Acquisition and analysis of mass spectrometry data was done essentially as described in (Hekmat et al. 2010; Rosengren et al. 2012), using a MALDI-TOF 4700 Proteomics Analyzer (Applied Biosystems, Framingham, CA) in positive reflector mode for data acquisition.
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Utilizing the superior mass resolution and accuracy of the Orbitrap mass analyzer, full scan mode was chosen for data acquisition in imaging mode rather than tandem mass spectra.
A mass scan range of 200 to 1,200 mass-to-charge ratio (m/z) was set for data acquisition in continuous mode with optimized parameters for ionization and mass transmission.
The notation used XXX/YYY (LTQ/LTQ or FT/FT or LTQ/FT or FT/LTQ) refers to the mode of data acquisition for MS and MS/MS spectra.
Data gateway should support multiple metering devices for data acquisition.
For data acquisition, the AQM acquisition manager program was used.
The acquisition cost is lowest for data acquisition and highest for knowledge and wisdom acquisition).
Fluoview software was used for data acquisition.
Empower software was used for data acquisition.
MR was responsible for data acquisition.
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