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Atomic force microscope (AFM) image was taken using Atomic Force Microscope (Park System, XE-70, South Korea) in a dark room at 25 °C in noncontact mode for confirmation of nanoparticle and conjugate size (Abdullin et al. 2009).
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The column oven was programmed from 80°C (3-min hold) to 250 °C at 15°C/min, followed by 250°C to 325°C at 5 °C/min, then held at 325°C for 5 min. A mass spectrometer (Agilent Technologies) was used in the single ion monitoring (SIM) mode at mass 270 and in the full scan mode for structural confirmation with an external standard method of quantitation.
A mass spectrometric detector (GC MS) in the selected ion monitoring (SIM) mode was also used for confirmation purposes.
UHPLC/ESI Q-Orbitrap Mass full scan mode acquired full MS data was used to identify and quantify additives, and data-dependent scan mode obtained fragment ion spectra for confirmation.
Acquisition took place in multiple-reaction monitoring mode, with two transitions per compound (one for quantification and one for confirmation) in positive ionization mode.
For confirmation of the dominant loss-of-function mode of action of the dSpt1 C129W mutant SPT subunit, we assayed the development of the larval NMJ.
They asked for confirmation.
"They were looking for confirmation".
Only seven Republicans voted for confirmation.
A line judge was summoned for confirmation.
I called a living naturalist for confirmation.
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