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"They will have to understand that we are in a research mode for at least the next year," he said.
To avoid the artifacts and uncertainties associated with an incomplete adaptation of the bacteria to carbon/energy sources, we ran batch cultures in a repetitive mode for at least 5 cycles.
Water containing 0.01, 0.1 and 1 wt.% SWCNHs and 0.03, 0.1 and 1 wt.% SDS, respectively, proved to be very stable even in static mode for at least 25 days.
Before samples were collected for fatty acid and pigment analysis (see below), cultures were acclimated to the different temperatures and irradiance levels in semi-continuous batch mode for at least one week; fast growing cultures were diluted with fresh medium to avoid nutrient limitation.
While recording, cells were injected with biocytin through recording pipette filled with internal solution containing biocytin in whole-cell recording mode for at least 30 min.
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In the subset of patients (8 treatments and 8 controls) ventilated in controlled modes for at least 24 h, we measured airways resistance daily, until switch to assisted modes of ventilation.
This represents a sizeable proportion of students that may be an interesting target for understanding how to intervene on travel practices to encourage a larger share of students to take active modes for at least one part of the daily school trip.
Experimental results showed that faceted crack initiation was the main failure mode for specimens at the stress ratios of −0.1, 0.1 and 0.5, and multi-site faceted crack initiation was observed at the stress ratios of 0.1 and 0.5.
These measurements allowed us to determine the dominant, intrinsic binding mode for SSB at each concentration of salt used in our single molecule experiments.
Isoelectric focusing was carried out in a gradient mode for 30 min at 500 V, 90 min at 1000 V, 90 min at 3500 V and 6 hours at 3500 V at 2 mA and 5 W.
It is rare for a start-up company working in a highly competitive and crowded market like mobile payments to stay in test mode for so long, at the risk of having rivals overtake it.
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