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The BWBBLE aligner was implemented in C. The program performs the indexing of and short-read alignment with a reference multi-genome; for convenience, it also provides a separate (faster) mode for aligning to a single genome.
When the total length of the spacer and the following sequence was >70 bases, the reads were aligned to target sequences (spacer + target region) with bwa (version 0.6.2) using the bwasw mode for aligning long reads and the parameter setting '-b5 -q2 -r1 -z10'.
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For all JAFFA modes, reads aligning to intronic or intergenic regions are first removed to improve computational performance (step 1 in Figure 1).
This simple model correctly predicts the red shift of the symmetric mode for end-to-end aligned dipole pair and the blue shift for side-to-side aligned dipole pair.
Figure 4 C shows the mode amplitudes for cells aligned parallel to the nanoridges (blue) and cells aligned perpendicular to the nanoridges (red).
Afterwards, both modes were aligned and joined in the same way as we did for the (SVO) and (SAV) samples.
using the EST2genome mode (DNA transcript aligned to genomic DNA)[ 79].
'Palindrome' mode aligns the forward and reverse reads, combined with their adapter sequences.
We filtered and aligned using paired-end mode for those tools that support it, but we used single-end mode as a fallback where necessary.
The 'local alignment' mode requires more computing time due to the dynamic programming, and is effective for aligning reads sequenced with adapters or continuous errors.
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