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To confirm the inhibitor binding mode and select the key drug-resistant amino acid residue, we determined the 2.2 Å co-crystal structure of DDR1 in complex with DDR1-IN-1, which confirmed the presumed type II binding mode.
Go to Image -> Mode and select Grayscale.
Go to Campus Legend mode, and select Use existing player.
Go to the Display Method (right next to Edit Mode) and select Wireframe.
Go to the layer tab and select mode and select 'Lighten Only'.
In the new image, go to Image -> Mode and select Grayscale.
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I put the T2i on Sports mode and selected continuous shooting, then pressed the shutter long enough to snap 20 shots.
The acquisition was controlled by an HP Vectra 48 PC chemstation computer in both full scan mode and selected ion mode (30ions 0.7cps 35 ms dwell) for greater sensitivity.
Sequences were automatically acquired by scanning first in MS mode and selecting the 15 most intense ions for MS/MS using an exclusion list of peaks arising from trypticautodigestion. Data interpretation was carried out with the GPS Explorer software (V3.5) and database searching with the Mascot program (version 2.0.00).
Electrospray-MS/MS was carried out on a Waters Quattro Micro tandem quadrupole (quadrupole-hexapole-quadrupole) instrument using a positive ionization mode and selected reaction monitoring (SRM).
Go to Image -> Mode again and select Bitmap.
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