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Putative contaminant sequences, are poorly represented (coverage ranging from 13 to 29 reads with a mean and modal coverage of 20 and 14 reads, respectively).
The Tyrolean Iceman's mtDNA, indeed, shows a coverage ranging from 19 to 1,030 reads, corresponding to a mean coverage of ∼153 and a modal coverage of 41 reads while the modern dataset displays a coverage spanning from 26 to 770 reads with a mean coverage of ∼204 and modal coverage of 52 reads.
We aligned ~10.1 Gb of genomic reads to the transcriptome assembly (see Methods), and estimated a modal coverage of ~24x per contig.
The modal coverage of the entire contig dataset was then used to estimate the depth to which the genome had been sequenced.
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Normalized coverage was determined by the log2 coverage within a 100 bp bin over the overall modal coverage.
The "cutoff" for each candidate was calculated from the number of purifications used for fingerprint analysis and based on a 10% coverage of a cellular protein with modal molecular mass, 50 kD.
Fig. 3a, 3b confirms that the samples with InAs coverage of 1.80 and 1.85 MLs have mono-modal size distribution whereas sample with InAs coverage of 1.95 MLs (Fig 3c) have bi-modal size distribution.
In this paper, however, we intend to take one step back and address the question from a theoretical perspective to (i) represent the multi-modal transportation system as a multiplex network; (ii) mathematically characterize the random walk coverage of this multiplex, and (iii) assess the robustness of such coverage when the system is confronted with failure.
Currently, one run of Roche-454 sequencing can produce one million reads, with a modal read length of 700nt - enough to sequence five 10kb viral genomes to a coverage of 20,000x each.
WORST: Coverage of S.E.
In this work, we propose MUME, an efficient algorithm for Multi-modal Urban Mobility Estimation, that takes advantage of the special structure of the supra-Laplacian matrix of the transportation multiplex, to compute the coverage of the system.
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