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The construct or empty vector (mock) were transfected to MDA-MB-231 and BT20 cells using lipofectamine 2000 (Life technologies) and clones were selected with G418 Lifee technologies).
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Control Jurkat cells were treated equally but were 'transfected' with medium alone, were mock-transfected using empty complexes (Mock), or were transfected with nonsense miRNA (Scrambled; Qiagen).
(b,c) Full length MDBK-derived MHC class I clones or a mock construct were transfected into HEK293 cells, stained with anti-bovine MHC class I mAb ILA88 as a positive control and IgG isolated from the serum of one (b) or four (c) BNP dam(s) and Ab binding was revealed using flow cytometry.
Mock samples were transfected with 35S::FER-GFP and empty SK vector DNA.
The CMV4-MAGEC2 expression expression vector and CMV4-flag mock vector were transfected into MCF-7 cells using Fugen HD (Roche).
To further characterize the functional interaction of uPAR with HSP70 and MRJ, HCT116 mock cells were transfected with selected amount of psiHSP70 or psiMRJ respectively.
In addition, the HCT116 mock cells were transfected with selected amount of psiHSP70 or psiMRJ alone or in combination or with 10 μM of the proteasome inhibitor, MG-132 (Sigma), for 8 hours in serum-free media.
Stable HEK293-P2X7R and HEK293-mock clones were transfected with an expression vector for the full length human P2X7R and an empty vector, respectively, as previously described.
For mock transfection, cells were transfected in the same manner, but without siRNA.
For mock transfections, cells were transfected only with pcDNA 3.1 hygro and EGFP.
Mock-treated and mock-depleted cells were transfected without the addition of dsRNA or with Rho1 dsRNA, respectively.
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