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Approximately 0.5 × 103 A549 cells transiently transfected with dsP21-322, scramble dsRNA and mock were plated in 100-mm culture dishes, respectively.
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These media were subsequently replaced with MEGM and an equal number of vHMECs from RM78 and RM79 overexpressing either TRF2, hTERT, vector (pWP), or mock infected were plated onto the top chamber of the transwell dish.
HCT116 cell lines (WT, mock and A/S) were plated directly onto the isotopically labelled collagen IV matrix at a density of 3 × 10 cells well−1 in 0.3 ml serum-free medium as described previously (Ahmed et al, 2002c).
Knockdown and mock-treated cells were plated at a subconfluent density and stained as normal for immunofluorescence and an image acquired at the same plane in each cell.
Mock and ICSBP cells were plated in a six-well culture plate.
NRP-1-transfected or mock transfected FG cells were plated at 1 million cells/10 cm dish and incubated with 1% FBS-MEM for 48 h.
Mock-transfected FG cells were plated at 60 70% confluence, and medium was changed to 1% FBS-MEM for overnight incubation.
TrkA or mock transfected NIH-3T3 cells were plated at 2 × 10 cells/well in 24-well plate for 24 h, washed and equilibrated in HKR binding medium for 30 min at 4° C.
In all, 1 × 10 (proliferation assay) or 1 × 10 (adhesion assay) mock and A/S HCT116 cells were plated on a specific protein-coated wells of a 96-well plate (Nunc, USA).
Pools of mock-transfected Rat-1 cells were plated in soft agar as a control.
Pools of mock-transfected HEK-293 cells were plated in soft agar as a control and showed relatively low number of colonies in soft agar (Figure 8B).
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