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Stable clones with HOPX or mock were established by G418 (GIBCO) selection (MIA Paca2, 800 μg/ml; PANC-1, 1200 μg/ml).
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GNG7 transfectants with pcDNA3-GNG7 (GNG7 expression vector) and mock cell lines were established in KYSE150 according to methods described previously (Shibata et al, 1999).
To determine whether the combination-induced apoptosis occurs in a COX-2-dependent route, COX-2 knocked-down cells (KD), which transfected COX-2 siRNA, and its mock control cells (Control) were established from MDA-MB-231 cells.
Stable CNE1 cells expressing si-mock or si-MSK1 were established with pcDNA6.0/myc-HisB as selection marker.
To separate the tumor growth-promoting effects from the Pa resistance-enhancing effects, the tumor weights of the Pa group where miR-193a-3p miR-193a-3p miR-193a-3ped tumor xenografts were established in the same individual mice were compared.
Cells transfected with the expression plasmid only were established as controls (mock transfectants).
Stable transfectants of SMMC7721-FXYD6 and SMMC7721-mock were established in the presence of 2 mg/mL G418. 1 × 105 cells were stained with FD10 (2 μg/mL) for 1 h at 4°C and followed by Alexa Fluor 488-conjugated anti-mouse secondary antibody for 45 min at 4°C.
Cells that stably harbour the corresponding empty vector (A549/Mock, H1299/Mock, and PC9/Mock) were established as controls.
After 60 days of screening, the cell lines stably expressing B4GALT1 (HL60/B4GALT1), B4GALT5 (HL60/B4GALT5) and empty vector (HL60/mock) were established.
After 4 weeks of screening, the cell lines stably expressing FUT4 (BEL7402/FUT6), FUT8 (BEL7402/FUT6), FUT8 (BEL7402/FUT8) and EV (BEL7402/mock) were established.
Based on the fission blanket, the uranium mock assemblies are established, and include the depleted uranium shell, the depleted-uranium/polyethylene/graphite shell and the natural uranium cube.
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