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Five-week-old Arabidopsis leaves were infiltrated with bacteria or 10 mM MgCl2 (mock) using a 1 mL needleless syringe.
For FB1 treatment, Arabidopsis leaves were infiltrated with 10 µM FB1 (in 10 mM MgSO4; Sigma, USA) or 10 mM MgSO4 (mock) using a 1 mL needleless syringe.
Ecotropic virus producer cells (Phoenix) were transfected with pMSCV-Myc-cdc6 or the empty vector (mock) using Lipofectamine™ 2000 Reagent (Invitrogen).
Each complex contained 10 µg of siRNA (for mock, using PBS instead of siRNA) and 7.5 µl Oligofectamine (Invitrogen) in PBS, which was mixed according to manufacturer's instruction of Oligofectamine.
PC3 cells were starved for 48 hours in androgen-deprived medium containing 10% dextran-filtered, charcoal-stripped fetal calf serum, and then transfected with pCMVhAR construct or vector alone (MOCK) using lipofectimineTM 2000 reagent (Promega Inc).
Significant differences were determined for each treatment between ABA and mock using t-test (p ≤ 0.05) and marked with asterisks.
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Mock used the opportunity to bring visibility to institutionalized problems affecting the transgender community.
K562 cells at 60 70% confluence were transfected with 1 μg of pCD-shRNA-MR-1 or pCD-shRNA-mock using 1 μl lipofectamine LTX Reagent (Invitrogen) for 2 days.
COS7 cells (1 × 10) were co-transfected with 8 μg each of Flag-tagged pCAGGSn3FH vectors (Flag-Bcl-GL and Flag-Mock) and HA-tagged pCAGGSnHC vectors (HA-WT-MELK, HA-D150A-MELK and HA-Mock) using the FuGENE 6 transfection reagent (Roche) according to the supplier's protocol.
Mocks are similar to stubs, but stubs use state verification, whereas mocks use behavior verification.
Figure 5c shows plasma membrane potential collapse of HEK293-P2X7R or HEH293-mock, used as positive and negative controls, respectively.
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