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Study design: We tested DBS from: (A) 39 laboratory reference cases; (B) 156 neonates suspected of having congenital CMV infection; (C) 119 children examined for the retrospective diagnosis of congenital CMV; (D) mock specimens prepared with known amounts of viral DNA.
Mock specimens were processed in parallel with patient specimens to monitor for reagent contamination.
Mock specimens (molecular grade water) were processed in parallel with patient samples to monitor reagent purity.
PCR cycling conditions were 95°C for 5 min followed by 30 cycles of 95°C for 30s, 55°C for 30s, 72°C for 1.5 min followed by a final cycle of 72°C for 10 min. All urine gDNAs yielded robust amplicons after 30 PCR cycles, whereas identically processed mock specimens (molecular grade water) failed to yield amplicons after 40 cycles.
Analytical sensitivity was estimated with surplus anonymized sputum specimens spiked before extraction with target bacterial species (mock specimens).
This was necessary for efficient extraction of Staphylococcus aureus DNA from sputum specimens, as we had previously found that mock specimens containing Staphylococcus aureus spikes were underquantified with standard automated extraction only.
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Furthermore, the volume fraction of the austenitic phase in the dual phase mock specimen was also successfully evaluated by fitting the diffraction pattern using a Rietveld code.
A pilot study conducted on three mock pipe specimens demonstrated that the process is effective on partially corroded and non-corroded pipes.
Data collected and processed from three laboratory mock-up specimens are presented and discussed, and further work is proposed.
The dsMock-treated specimens (n = 8) produced normal eggs whereas those treated with dsBrownie-2 (n = 12) produced eggs with a malformed sponge-like body, exactly as in the specimens treated with dsBrownie-1 (Figure S5).
"Full fabrication of the curtain wall cannot begin until the mock-up specimen passes these tests".
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