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Subsequently, plants were transferred to LD growth conditions, watered with 15 µM Dex or Mock solution and incubated until harvest of second internodes for RNA isolation.
For additional short-term CHX treatment, 10 mM CHX stock was added to the 15 µM Dex and Mock solution to a final concentration of 10 µM and the plants were treated in the same way as described before.
To examine an immediate effect of bikinin treatment in in vivo phosphorylation assay and gene expression analysis, siliques collected at 4 days after pollination were treated in bikinin or mock solution for 2 h.
For short-term Dex treatments 15 20 cm tall plants were dipped headfirst in 15 µM Dex (25 mM Dex stock diluted in tap water + 0.02% Silwet) or Mock solution (equal amount of 100% Ethanol in tap water + 0.02% Silwet) for 30 s.
Plants were then transferred to LD conditions and watered twice a week with either 15 µM Dex (25 mM Dex stock diluted in tap water) or mock solution (equal amount of 100% Ethanol in tap water) until they reached a height of 15 20 cm and were harvested for histology.
One hour after the start of the photoperiod, 1 ml 100 µM MeJA (Serva, Heidelberg, Germany) in 0.1% ethanol or mock solution with 0.1% ethanol only, were applied to the filters.
Similar(39)
We applied a four-factor at two-level factorial design with central point to synthesize forty mock solutions spiked with two pathogen surrogates, Salmonella Typhimurium and bacteriophage PP7, selected as bacterial and viral models, respectively.
Mock solutions only contained ethanol (1‰).
Mock solutions contained the appropriate solvent.
Mock solutions for the different experiments were made using NaOH as to keep pH constant.
Each, though, is essentially a book within a book, a gloss on a great deal of bygone literary criticism and a mock-solution to the uncrackable mystery of Dickens's last novel.
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CEO of Professional Science Editing for Scientists @ prosciediting.com