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Changes in [Ca2+]i mobilization were determined by measuring peak rises in [Ca2+]i mobilized (Δ[Ca2+]i) and were calculated as mean changes in Δ[Ca2+]i ± S.E.M. (nM).
To gain more insights into the dynamics of oil and starch reserves and find conditions that will be useful to screen for mutants impaired in oil degradation, experimental conditions causing TAG breakdown were sought and kinetics of mobilization were determined.
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Receptor desensitization assay based on Ca2+ mobilization was determined as described previously [49].
SDF-1-regulated cell mobilization is determined by local SDF-1 gradients and/or CXCR4 expression on target cells.
Range of motion of the hip, knee and ankle, ligament stability of the knee, as well as current mobilization status were determined at the follow-up examination.
We did not record mobilization data, and pain scores were determined only at rest.
Receptor signalling and function were determined by mRNA knockdown and intracellular calcium mobilization experiments.
Relative importance of Fe mobilization agents was determined by comparing their influence coefficients for the considered time period.
The level of mobilization should be determined by the patient's strength and endurance, as well as an assessment of the safety criteria.
Cells were then washed and resuspended in 1.5 ml of the same buffer and time course of Ca2+ mobilization (0 5 min) was determined using Hitachi F-2500 Fluoro spectrophotometer (San Jose, CA) with an excitation wavelength of 355 nM and an emission wavelength of 410 nM [9].
The precise cellular target remains elusive and whether these protective molecules influence all progenitor cell differentiation and mobilization remains to be determined.
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