Genes assigned putative functions in replication and mobilization were identified on all plasmids along with open reading frames (ORFs) of unknown function, many of which exhibited no significant homology to sequences present in the databases (Fig 1, Table S1 in additional file 1).
Within the same contig, an origin of transfer (oriT) was identified based on the 99% similarity (expect = 0.0) to the mobilization region of pAD1 where an oriT has been previously identified between ORF53 and ORF57 (AF343837) [24].
At present, the following loci involved in the plasmid vegetative replication and its mobilization, have been identified in RSF1010 [ 16, 17] (see Fig. 1 as well): 1) Transcription of all corresponding genes is initiated from the promoters (P1 – P4) (see Fig. 1).
Recently, two large series of patients with lymphoma or MM have been retrospectively evaluated define PM, according to the standardized criteria: extensive previous chemotherapy, previous melphalan exposure and a previous failed mobilization attempt have been identified as predictive factors of poor mobilization.
Double strand origins, putative single strand origins and typical mobilization start signals were identified.
Protocols and publications that outlined safety criteria for early mobilization in ICU were identified and distributed to the group.
All the elements necessary for plasmid mobilization in Gram-positives were identified in pLG2 as can be seen in Table 3.
Of ∼20,000 progeny scored, three candidates were identified from mobilization in the female germline and none from mobilization in the male germline, based on mobilization of the Hsp70- miniwhite marker to the third chromosome.
Two open-reading frames that code for proteins involved in plasmid mobilization and initiation of replication were identified.
