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GCP-WD is phosphorylated in mitosis, resulting in a mobility shift in SDS-PAGE [13], [14].
Cdc24 displays little to no mobility shift in the Δbem1 strain [12], [17].
p7-IQSPHFF-containing phage clones were positively screened by mobility shift in PCR and confirmed by DNA sequencing.
Using phosphate-affinity gel electrophoresis [37], Borealin displayed a prominent mobility shift in mitotic samples of nocodazole-treated cells (Fig. 6D).
Mobility shift in each mutant strain was quantified and normalized to an appropriate wild type strain resolved on the same gel.
XDpr1aΔMTTV lacks the PDZ-B domain and did not undergo a mobility shift in the presence of CKIδ, suggesting that it is not phosphorylated by CKIδ (Fig. 3A, compare lane 6 with lane 5).
XDpr1a exhibited a mobility shift indicative of hyperphosphorylation in the presence of XDsh and ***β-βXDsh (Fig. 3B, compare lanes 2 and 3 to lane 1), whereas XDpr1a did not exhibit a mobility shift in the presence of *αXDsh (Fig. 3B, lane 4 compared to lane 1).
Although we did not find significant changes in PBP serum levels of disease subjects compared to controls, the acid form of PBP suggests that the PBP mobility shift in 2D maps might be related to PBP post-translational modifications, e.g. protein phosphorylation state, more than to PBP quantitative differences (Table 3, Table S1) [34].
We note that sorafenib and 885-A induce a mobility shift in BRAF in SDS-gels.
Conversely, Bid containing a substitution at S66 alone (BidYFP-S66A) showed no mobility shift in mitosis.
BRAF also undergoes a mobility shift in PLX4720 treated cells in the presence of PD184352.
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