Sentence examples for mobility shift in from inspiring English sources

Cdc24 displays little to no mobility shift in the Δbem1 strain [12], [17].

GCP-WD is phosphorylated in mitosis, resulting in a mobility shift in SDS-PAGE [13], [14].

p7-IQSPHFF-containing phage clones were positively screened by mobility shift in PCR and confirmed by DNA sequencing.

Using phosphate-affinity gel electrophoresis [37], Borealin displayed a prominent mobility shift in mitotic samples of nocodazole-treated cells (Fig. 6D).

Mobility shift in each mutant strain was quantified and normalized to an appropriate wild type strain resolved on the same gel.

XDpr1aΔMTTV lacks the PDZ-B domain and did not undergo a mobility shift in the presence of CKIδ, suggesting that it is not phosphorylated by CKIδ (Fig. 3A, compare lane 6 with lane 5).

We found mobility shifts in the PCR products from two of the patients and none of the controls.

As shown by mobility shifts in gel electrophoresis, the PBL1 protein, encoded by the CCE5 gene, is apparently phosphorylated in vivo after MAMP elicitation [ 34].

The SSCP technique is based on conformational differences of single stranded DNA fragments that can be detected as mobility shifts in non-denaturing polyacryilamide gel electrophoresis [ 15].

Where indicated, Phos-tag (Wako Chemicals, Japan) and MnCl2 were added into 10% gels to induce mobility shifts in phosphorylated proteins, to final concentrations of 40 µM and 80 µM, respectively, for Figure 5B, Figure 5 figure supplement 1A and Figure 7 figure supplement 1C, or 25 µM and 50 µM, respectively, for Figures 5D,E,7G.

DOI: http://dx.doi.org/10.7554/eLife.05828.014 As bZIP63 expression is strongly regulated by the day/night cycle and by sugars (Matiolli et al., 2011; Kunz et al., 2014), we investigated its phosphorylation status under these conditions, applying the Phos-tag technique to enhance phosphorylation-induced mobility shifts in 1D SDS-PAGE (Kinoshita et al., 2006).