Sentence examples for mobility shift analysis from inspiring English sources

We confirmed that the ER antagonist activity of tRA may be mediated by transcriptional interference after ER ligand complex binding to an estrogen responsive element of the gene by the gel mobility shift analysis.

(D) The sequences of CTCF binding motif 2. (E) Gel mobility shift analysis of GST-CTCF-ZFs protein with M2 motif.

Electrophoretic mobility shift analysis demonstrated that the site at -3306 generated a specific protein-oligonucleotide complex that was supershifted by C/EBP alpha antibody, while a probe corresponding to a putative site at -1462 did not.

(F) Gel mobility shift analysis of competition of GST-CTCF-ZFs binding to the biotin-labeled M2 by an excess amount of unlabeled M2. Assay condition was the same as in Fig. 1B.

Five of the RARE half-sites had the minimal spacer nucleotide requirement for functionality and DNA mobility shift analysis with different pairs of the RARE half-sites and supershift studies using antibodies against RAR-beta showed significant binding of nuclear protein complexes from RA-treated cells to these RAREs.

Using EMSA (electrophoresis mobility shift analysis), Örd et al. [29] demonstrated that ATF4 binds in vitro the TRB3 AARE in response to ER stress.

The electrophoretic mobility shift assay analysis was done essentially as described by Shiraga et al. [37].

The concomitant electrophoretic mobility shift assay analysis for the evaluation of the NF-κB DNA binding confirmed an increased binding in E2-treated cells, when compared with other conditions with or without staurosporine, as shown in Fig. 6a.

DNA methylation analysis, electrophoretic mobility shift assay (EMSA) analysis, identification of a Monkey King-related microRNA (miRNA), and transgenic analysis revealed its effects on gene expression and genome evolution in Brassicaceae.

PCR products that revealed mobility shift on SSCP analysis were send for DNA sequencing.

Electrophoretic mobility shift assay (EMSA) analysis and signal quantification were performed as described (Hartl et al., 2010).