Sentence examples for mobility shift analyses from inspiring English sources

(C) Gel mobility shift analyses of competition of GST-CTCF-ZFs binding to the biotin-labeled M1 by an excess amount of unlabeled M1.

Open image in new window Figure 1 CTCF-ZFs bind to M1 stronger than to M2. (A) The sequences of CTCF binding motif M1. (B) Gel mobility shift analyses between M1 motif and increased amount GST-CTCF-ZFs fusion protein.

This Myc2 proteintein was then tested together with the purified Myc1 p16 (amino acid residues 201-314) and the purified full-length Hydra Max protein (Fig. 4B) (Hartl et al., 2010) using electrophoretic mobility shift analyses (EMSA) (Fig. 4C).

γ-Glutamylcysteine synthase, the rate-limiting enzyme of glutathione biosynthesis, is stimulated by melatonin at the transcriptional level; EMSA (electrophoretic mobility shift analyses) data have shown melatonin-dependent rises in DNA binding not only of AP-1, but also of RZRβ/RORα [ 79].

Early SELEX (systematic evolution of ligands by exponential enrichment) and EMSA (electrophoretic mobility shift assays) analyses demonstrated that recombinant FUS selectively binds RNAs containing a GGUG motif with nanomolar affinity in vitro (Lerga et al., 2001).

Furthermore, NF-κB DNA biNF-κB DNAn anti-CD3/CD28 treatment was strongly reduced in Coro1a-deficient T cells when analysed bindingtrophoretic mobility shift assay (EMSA).

The DNA substrates were incubated with increasing amounts of Srs2ΔC, and the reaction mixtures were analysed by electrophoretic mobility shift assay (EMSA).

Nuclear extracts were prepared and analysed by electrophoretic mobility shift assay (EMSA) using the m67 high-affinity STAT-binding site probe, as described previously (Ward et al, 1999a).

The binding activity of PdhR towards the binding sites in front of the genes glcD, mraZ and metB were also analysed by electrophoretic mobility shift assays.

The STAT3 DNA binding was analysed by electrophoretic mobility shift assay (EMSA) using a P-labelled high-affinity sis-inducible element (hSIE) probe (5′-CTTCATTTCCCGTAAATCCCTA-AAGCT-3′ and 5′-AGCTTTAGGGATTTACGGGAAATGA-3′) as previously described (Bhutani et al, 2007).

Analyses of an electrophoretic mobility shift assay and a reporter gene further showed that LPS increased the transactivation activity of AP-1 in A549 cells.