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Preliminary studies were conducted with a number of different compounds predicted to inhibit planarian mobility (data not shown).
In fact, double treatment of HeLa extracts with 2Apro and HIV-1 PR renders an eIF4GII C-terminal polypeptide similar to that found with 2Apro alone, whereas the N-terminal fragment increased its electrophoretic mobility (data not shown).
Instead, the concentration decrease was owing to a fast loss of mobility (data not shown), whereas only moving cells were counted with the Thoma chamber.
Western blot analysis of mdmx in presence or absence of pCLPGp19, doxorubicin or nutlin-3 did not reveal any alteration in protein level or mobility (data not shown).
SDS-PAGE of metabolically labelled types I, III, and V (pro collagen secreted by and retained in fibroblasts of individual II.5 did not show qualitative or quantitative abnormalities in electrophoretic mobility (data not shown).
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HP0593 Y107G and HP0593 C54G mutant proteins were purified to near homogeneity and analyzed on SDS-polyacrylamide gel wherein there was no change in mobilities (data not shown).
It is worth specifying that symptoms and mobility data sets do not completely overlap.
Like ethanol, these agonists increased the lateral mobility of MOP (data not shown).
Mutants deleted for CTK1 did not alter the electrophoretic mobility of Not4p (data not shown), indicating that this CDK is not the required kinase for Not4p.
Interestingly, yeast mutants deleted for PHO85 showed an increased electrophoretic mobility of Not4p (data not shown), suggesting that Pho85p is involved in Not4p phosphorylation.
However, the mobility of macrophage (data not shown) and the change of phenotype toward non-M2-like macrophages were found to be decreased.
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