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More local mobility corresponding to the β and γ relaxations is also modified, but in a lesser extent.
Analysis of the D of remaining QDs revealed increased fractions of confined/slowly diffusing receptors and of highly mobile/non-confined receptors at the expense of QDs with "intermediate" mobility, corresponding to extrasynaptic α7-nAChRs (example 3 in Fig. 3) (Kolmogorov-Smirnoff; pvehicle/latrunculin A<10−3, pnocodazole/latrunculin A<10−3).
A double mutation C94/99A ran as a single band with a mobility corresponding to the slowest band in the single mutants C94A and C131A.
The pure enzyme is a monomer with an electrophoretic mobility corresponding to the size ≈27 KDa, as expected from its sequence.
Although the M1X band had a lower intensity with respect to the wild type TMEM16A band, it had similar electrophoretic mobility corresponding to ~ 120 kDa.
The rabbit polyclonal antibody detected a single major protein with an electrophoretic mobility corresponding to that of the major protein detected by the monoclonal anti-sialophorin antibody L10 (Supplementary Figure 2; Remold-O'Donnell et al, 1984).
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The propellant protons can be schematically divided into three segmental mobilities corresponding to distinct relaxation times.
Triple-myc epitope-tagged Ste7 and Tec1 proteins showed gel mobilities corresponding to estimated molecular weights of 72 kD and 73 kD, respectively.
In the absence of added Ca2+, Aβ(1 40) molecules had aggregated to such an extent that we were able to detect only bands with low electrophoretic mobility corresponding mainly to high-molecular-weight oligomers, protofibrils and fibrils, located in or near the stacking part of the polyacrylamide gel (Fig.1A, two last lanes).
Phase I contains polymer chains with a mobility similar to that in the purely amorphous polymer, while Phase II shows a much more restricted mobility, probably corresponding to conformational changes within the intraspherulitic regions.
The immunoblots also showed an increase in higher mobility bands corresponding to FUS oligomers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com