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The rate constant of hybridization could be slower for hybridization of mobile strands to immobilized strands (kf-bound) on a solid surface and values in a range up to 100-fold lower than kf have been used in simulations.
Tie mobile strands to the base.
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An analytical solution for the concentration distribution of mobile strands at intermediate hybridization times provides a convenient tool to calculate the mobile strand concentration profiles.
It has been shown before that only the mobile strands within a radius of 0.15 cm from the spot are available for hybridization due to transport effects [ 12].
However, the rate constants for reaction between two mobile strands may differ from the rate constant for the reaction of a mobile strand and a bound strand.
The immobilized strands are distributed uniformly in the spot phase and the mobile strands are present both in the solution phase and spot phase.
At low values of the rate constant for hybridization between immobilized and mobile strands, the assay performance varies with the hybridization time and initial cDNA concentrations.
String mobile strands.
The assay involves hybridization of mobile DNA strands with immobilized complementary DNA strands to form duplexes.
For example, you could anchor each mobile strand with antique doorknobs or oversize buttons.
BAA has several strands to its argument.
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