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The chromatographic column from stainless steel comprised the stationary stage octadecylsylane (ZORBAX Eclipse XDB-C8 250∗4.6 mm∗5 μm); mobile stage: methanol : water (95 : 5), v/v; flow rate of the mobile stage: 1.2 mL/min; detective: 280 nm; injection volume: 20 μL.
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The mobile phase (methanol-water mixture) had a flow rate of 1.1 mL/minute.
February 7 on Scene Videotron Mobile Stage.
Mobile phase B: methanol.
The mobile phase was methanol and H2O with an isocratic gradient (50/50).
The mobile phase was methanol: IPA 70 30.
The mobile phase was methanol and water.
The mobile phase was methanol with a flow rate of 1.00 mL/min.
Mobile phase water-methanol (99 1, v/v) 0.1% HFBA.
Mobile phase water-methanol (99 1, v/v) 0.1% TFA.
Samples then were separated with solid phase extraction (SPE) column (LiChrolut RP-18 1000 mg/6 ml, Merck Germany) with mobile phase of 60% methanol and 70% methanol.
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