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Whereas most puncta were stationary, we observed mobile puncta containing both YFP-Kv1.2 and CFP-Kvβ2 moving in either anterograde or retrograde direction (Fig. 3A), as well as mobile puncta containing only one of them.
While most puncta were stationary, mobile puncta containing both of them were observed in both anterograde and retrograde directions (Figure S4E).
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Approximately, one third of mobile puncta contained both Kv1.2 and EB1, and those puncta traveled over longer distances in both directions (Fig. 3D).
Formation of autophagosome puncta containing LC3 has been regarded as a hallmark of autophagic activation.
The length and linear density of active zones, and the numbers of perisomatic puncta containing inhibitory axonal markers, are also increased (Nikonenko et al., 2006).
Cells were malformed and in some cases puncta containing BiP together with swollen ER were found.
In the retrograde direction, puncta containing both also moved faster (Fig. 3C).
The puncta containing both also moved over longer distances compared to those containing only one (Fig. 3D).
Finally, using quantitative microscopy, we estimated the number of YFP-Kv1.2 molecules in different puncta containing YFP-Kv1.2 YFP-Kv1.2
Compared to the plus-end tracking of EB1-YFP, the puncta containing both CFP-Kvβ2 and EB1-YFP moved faster in both directions (Figure S4A D).
To do so, we examined if βcat was retained in puncta containing these mutants.
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