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For two mobile phases with equal salt molarity, the mobile phase with the lower pH provided a greater peak area.
HILIC is a chromatographic method that utilises hydro-organic mobile phases with a high organic content, and a hydrophilic stationary phase.
Then the separations of different classes of compounds such as amphenicol antibiotics, macrolide antibiotics or basic test solutes with mobile phases with pH up to 10.8 are described.
The model parameters were evaluated using a rather large set of retention data measured in mobile phases with various counterions and acetonitrile concentrations.
When comparing two mobile phases with equal pH, the mobile phase with the lower salt molarity resulted in a greater peak area.
These compounds were chromatographed on octadecyl silica (ODS) stationary phase and their hydrophobic parameters expressed as the log kw values were determined by RP-HPLC, using mixtures of methanol and water as mobile phases with different methanol concentrations.
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The most important factors were the column temperature, with lower temperatures giving better resolution, and pre-column mixing volume of sample with mobile phase, with higher mixing volumes giving better resolution up to an asymptote reached at around 150 μl.
Optimum condition for separation of flavonoids was as follows: methanol:0.4% phosphoric acid in water:THF (45.3 54.4 0.3, v/v/v) as mobile phase with flow rate of 1 mL min−1 at 30 °C.
The drugs were extracted by the mobile phase with shaking and filtration.
Thin layer chromatography was carried out using chloroform: methanol mobile phase with a composition of 95 5.
In addition, mobile phase with high organic solvent ratio increased the ionization efficiency of LS.
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