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The selection of mobile phases was initiated with traditional alkane/alcohol mixtures (Chiral Technologies 2004).
Formic acid was tested at 0.1% with different ratios of acetonitrile; the pH of these mobile phases was 3.1.
Moreover, peak broadening and overlap of [18F]AV1451 2 with contaminants, that occurred when we used acidic HPLC mobile phases, was not an issue with the basic buffer.
This has led to the design, construction and application of a toroidal column fitted to the Brunel Dynamic Extraction Centrifuge, providing pseudo-hydrostatic CCC in which much improved mixing for the stationary and mobile phases was obtained.
A new green thin-layer chromatographic system comprising ionic liquid (1-methylimidazolium chloride) impregnated silica gel G and 2-methyl tetrahydrofuran as stationary and mobile phases was found to be most suitable for the separation of ternary mixture of biosurfactants (sodium cholate, sodium deoxycholate and sodium taurocholate).
This was performed with peptides from 2 mg protein in replicate as previously described except that the pH of the mobile phases was 2.7, not 3.0 [3.0.
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The mobile phases were prepared accordingly.
Two different mobile phases were employed in the assay procedure.
Mobile phases were degassed using Merck L 7612 solvent degasser.
The mobile phases were filtered prior to use.
Three mobile phases were used.
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