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The workflow developed allowed the use of acidic mobile phases for the first time.
Spectra were acquired in the suitable solvents i.e. the one used as mobile phases for the HPLC analyses.
Then, samples were lyophilized (Thermo Scientific Heto PowerDry) and the final extract was resuspended in 0.1 mL of a 50 50 (v:v) mixture of A B mobile phases for HPLC analysis.
Enhanced fluidity (EF) liquid mixtures are advantageous as mobile phases for the separation of moderate to polar compounds in liquid chromatography (reversed-phase, normal, size exclusion, size exclusion, and chiral separations).
In the present study, the polyaniline-modified silica gel nanocomposite (Pani@SG-S2) has been prepared by in situ polymerisation of aniline to be used as stationary phase with several aqueous ionic liquid mobile phases for thin-layer chromatographic analysis of six dyes, viz.
Mobile phases for gradient elution were 0.1% acetic acid aqueous solution (HOAc aq)) : acetonitrile (ACN) = 60 : 40 at 0 10 min and 0.1% HOAc aq) : ACN = 50 : 50 at 10 23 min.
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We were interested to develop a common mobile phase for all pyrethroids under study.
Sharp peaks were obtained with a presaturation of mobile phase for 20 min.
The mobile phase for the determination of MMC was water-methanol (65/35, v/v).
The mobile phase for the substance atrazine consisted of 68.5% water and 31.5% acetonitrile.
The optimum composition of mobile phase for the separation of the eight isoflavones was obtained.
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