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The mobile phase consisted of a linear gradient with two mobile phases containing acetonitrile and water.
The dependence of the temperature and the pressure drops on the Joule Thomson coefficient and kinematic viscosity are described for carbon dioxide mobile phases containing up to 20% methanol.
Indeed, the improved ESI sources equipping the recent mass analyzers allow for enhanced evaporation efficiency, mainly for RPLC mobile phases containing high proportion of water and this even at high flow rates.
The test mixtures were separated on a total of twelve different column chemistries at three different temperatures, using CO2-methanol-based mobile phases containing a variety of polar additives.
The polar hydro-organic (55/45) mobile phases containing an aqueous solution adjusted to pH = 3.7 and an organic solvent (MeOH) including triethylamine (TEA) and trifluroacetic acid (TFA) are used.
The LC mobile phases containing 0.1‰ formic acid was helpful to improve the peak tailing of flavonoids.
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These mobile phases contain many of the important physicochemical parameters of alveolar surfactant.
Both mobile phases contained 250 μL of 1.44 mM sodium dodecyl sulfate and 30 μL of perchloric acid.
The mobile phases contained either 10% ACN, for ordinary AEX operation, or 70% ACN, where a significant degree of hydrophilic interaction was superimposed upon the electrostatic effects.
All the experiments were carried out at a flow rate of 0.5 mL/min, protein mobile phases contained between 15 mg/mL and 5 mg/mL of protein and the injection size was 10 μL.
The best separation was obtained with a mobile phase containing 50 mM phosphate, pH 5.5 and 6% methanol.
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