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The gradient mobile phases consisted of (A) water containing 0.05% formic acid and (B) acetonitrile for gradient elution from the column at 40°C.
The mobile phases consisted of eluent A (0.1% formic acid in water, v/v) and eluent B (0.1% formic acid in methanol, v/v).
Mobile phases consisted of 0.1% formic acid (A) and MeOH (B) with isocratic elution at 50 50 (v/v) at a flow rate of 0.4 mL min-1 for 1 min. Electrospray ionization (ESI) was performed in positive ion mode from m/z 100 to 700 using the linear ion trap (LIT) operating under enhanced MS (EMS) mode.
The separation was conducted on a Waters Acquity UPLC BEH C18 column (1.0 × 150 mm with 1.7-μm particles), and the mobile phases consisted of A: H2O and 0.1% formic acid and B: 90% acetonitrile, 9.9% H2O and 0.1% formic acid.
The mobile phases consisted of 0.1% formic acid in water (Solvent A), and 0.1% formic acid in acetonitrile (Solvent B).
Mobile phases consisted of solvent A, 0.1% TFA in water, and solvent B, 0.1% TFA in acetonitrile.
Similar(13)
The mobile phase consisted of water (A) and methanol (B).
The mobile phase consisted of methanol water (70 30, v/v).
The mobile phase consisted of acetonitrile and water (90 10).
The mobile phase consisted of a gradient eluting system.
The mobile phase consisted of 0.001 N sulphuric acid.
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