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Different mobile phases and pHs ranging from 2 11 were tested.
Various mobile phases and differing gradient compositions were explored for optimal results with the final selected parameters as follows.
It illustrates the similarities and differences in the pressure and density profiles along columns operated with mixed mobile phases and with neat CO2.
Synthesis protocols for d-[18F]FAC and l-[18F]FMAU (Figure 7) were nearly identical, differing only in precursors and HPLC mobile phases, and were programmed using the ELIXYS drag-and-drop software interface [20].
Several inorganic, organic and aqueous-organic mobile phases and different stationary phases have been used in TLC analysis of surfactants (Mohammad and Agrawal 2001; Mohammad et al. 2002; Touchstone 1992; Gocan 2002).
Consequently the retention mechanism of the target enzyme on the AAILs can be as either in typical hydrophobic or ionic chromatographic supports, so long as selecting various mobile phases and separation conditions.
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Dried samples were reconstituted with the mobile phase and.
Actually, solvents acetonitrile and methanol used for mobile phase and extraction were of UPLC-MS grade.
The residue was reconstituted in 80 μl mobile phase and analyzed using HPLC.
Aqueous solution of methanol was used as mobile phase and the injection volume was 5 μl.
The solutions were diluted to the mark with the mobile phase and mixed well.
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