The drugs were extracted by the mobile phase with shaking and filtration.
In addition, mobile phase with high organic solvent ratio increased the ionization efficiency of LS.
The mobile phase with the extracted racemic malathion was evaporated at reduced pressure to isolate the racemic malathion.
Acidic mobile phase with a buffer of pH 3.2 was chosen to protonate nitrogen atoms for a fast elution.
Two solvents with a gradient were used as mobile phase with a flow rate of 1 mL/min.
Thin layer chromatography was carried out using chloroform: methanol mobile phase with a composition of 95 5.
For two mobile phases with equal salt molarity, the mobile phase with the lower pH provided a greater peak area.
Cordycepin and adenosine were separated by gradient elution using the mobile phase with the composition of water methanol (95.0/5.0 to 58.4/41.6 in 20 min, v/v).
Acetonitrile/water (containing 0.05 % acetic acid glacial) (60 40, mL/mL) was used as the mobile phase with a flow rate of 1 mL/min.
Acetonitrile of HPLC grade (JT Baker) and trifluoroacetic acid (Mallinckrodt) were used for the mobile phase, with a minimum purity grade of 99%%.
The mobile phase, with a total rate of 300 μl/min in gradient mode, was composed of water and methanol, each containing 0.2%% formic acid.
