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Mobile phase velocity decreased with the addition of Nafion into the electrospinning solutions.
In order to increase mobile phase velocity and thus the speed of analysis the availability of voltages higher than 30 kV was accomplished in some of these devices.
The variation in mobile phase velocity as a function of the column radius has been shown to be a major limitation in the efficiency of HPLC columns.
These columns reveal a retention-insensitive plate height in the separation of small retained molecules which only slightly decreases at increased linear mobile phase velocity.
Chromatography column: ZORBAX Eclipse XDB-C18 4.6 × 150 mm, mobile phase: 1% HAc-MeOH (1 1, v/v); mobile phase velocity, 0.8 ml/min; column temperature, 30°C; sample volume, 20 μl; wavelength of ultraviolet detector, 277 nm.
While the water bath minimised axial temperature gradients and thus its effect on k, radial temperature gradients were potentially serious with this system, especially at high mobile phase velocity, even with columns containing 5 μm particles.
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High resolution and fast separations are achieved since the small particles allow good efficiency with fast mobile phase velocities (one centimetre per second or higher).
The efficiency, the mass transfer, and the optimal velocity increase simultaneously with increasing temperature, which enables the use of high mobile-phase velocity.
The elevated mobile phase linear velocity results in high resolution, sensitivity, and shorter analysis time.
A maximum mobile phase linear velocity was found to depend on the centrifugal field for the 30-mL rotor.
This monolithic column (referred to as naphthyl methacrylate monolithic column or NMM column) showed high mechanical stability at relatively high mobile phase flow velocity indicating that the column has excellent hydrodynamic characteristics.
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