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The stock solutions were further diluted with the mobile phase to obtain the working concentration ranges.
The stock solution was further diluted with the mobile phase to obtain the working standard solution.
Trials were made to choose a proper mobile phase to obtain maximum resolution and peak symmetry.
Two pairs of pumps were used for each mobile phase to create gradient elution capability.
One milliliter WS1 was diluted tenfold using mobile phase to prepare working standard 2 (WS2, 10 µg/mL).
One mL of WK1 was diluted 10-folds with mobile phase to give working solution 2 (WK2, 10 µg/mL).
The components will be carried along with the mobile phase to varying degrees, and a separation will be achieved.
Subfraction FG11 was subjected to CC on Sephadex LH-20 using MeOH as a mobile phase to give 9 subfractions.
LiBr (40 mM) was added to the DMF mobile phase to dissociate molecular aggregates of polymer during GPC analysis.
An exact amount was subsequently prepared as dilutions in the optimized mobile phase to make a set of calibration and quality control solutions.
One mg/mL stock solution of VNT was prepared in DMSO then diluted 10-folds with the mobile phase to give working solution 1 (WK100100 µg/mL).
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