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HMF, HMF alcohol, and HMF acid were analyzed at the mobile phase rate of 0.6 mL/min, and the detector wavelength was 230 nm.
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Three parameters were varied: detector temperature, gas flow rate (nebulizer), and mobile phase flow rate (See Figure 2), and their effect on the Signal/Noise (S/N) ratio was monitored.
Percentage of acetonitrile in mobile phase, flow rate and pH; an increase in the flow rate results in a decrease of the drug found concentration, while the percentage of organic modifier and pH have no important effect on the response.
The mobile phase flow rate was 0.30 mL min-1; to study the effect of the flow rate on resolution, the flow rate was changed to 0.27 and 0.33 mL min-1.
The mobile phase flow rate was 1 mL/min.
The mobile phase (flow rate 0.25 mL/min) was methane sulfonic acid.
Potassium hydroxide was used as the mobile phase (flow rate 0.30 mL/min).
The mobile phase flow rate was maintained at 0.2 ml/min during the run time.
A mobile phase flow rate of 0.8 mL min−1 was employed at 25 °C.
The mobile phase flow rate was 2.0 mL/min and the injection volume was 20 μL.
In all the experiments, mobile phase flow rate was kept constant at 5.0 L/min.
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